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manP [2018-02-13T09:50:41.000Z]
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manP [2018-02-13T09:50:41.000Z]

trigger enzyme: mannose-specific phosphotransferase system, EIIBCA of the PTS
Locus
BSU12010
Isoelectric point
6.72
Molecular weight
61.81 kDa
Protein length
589 aa Sequence Blast
Gene length
1767 bp Sequence Blast
Function
mannose uptake and phosphorylation, control of ManR activity
Product
trigger enzyme: mannose-specific phosphotransferase system, EIIBCA
Essential
no
Synonyms
yjdD
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → Phosphotransferase system → Sugar specific PTS proteins
  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of mannose
  • Information processing → Regulation of gene expression → Transcription factors and their control → Control of PRD-type regulators
  • Information processing → Regulation of gene expression → Trigger enzyme → Trigger enzymes of the PTS that control the activity of PRD-containing transcription factors
  • Groups of genes → Membrane proteins
  • Groups of genes → Phosphoproteins → Phosphorylation on a Cys residue
  • Groups of genes → Phosphoproteins → Phosphorylation on a His residue
  • Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue

    • Gene

    Coordinates
    1,272,725 → 1,274,677

    The protein

    Catalyzed reaction/ biological activity

  • transport and concomitant phosphorylation of mannose

    • Protein family

  • PtsI permease, fructose/ mannitol permease (Fru) family PubMed

    • Paralogous protein(s)

  • FruA

    • Modification

  • phosphorylation on Ser-365 PubMed

    • Structure

  • 2R48 (IIB domain)

    • Localization

  • membrane

    • Expression and Regulation

    Operons

    Genes
    manP-manA-yjdF
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • ManR: activation, in the presence of mannose and absence of glucose PubMed, in ManR regulon
  • RNA switch: termination/antitermination, expession may be controlled by a potential RNA switch located in the 5' untranslated region of the manP-manA-yjdF mRNA between manA and yjdF, in RNA switch

    • Regulation

  • induced by mannose (ManR) PubMed
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    Biological materials

    Mutant

  • MGNA-A270 (yjdD::erm), available at the NBRP B. subtilis, Japan
  • BKE12010 (ΔmanP::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGGAAACCTCCTTTAAA, downstream forward: _UP4_ATCGAATAAAGCGGGGGATT
  • BKK12010 (ΔmanP::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGGAAACCTCCTTTAAA, downstream forward: _UP4_ATCGAATAAAGCGGGGGATT

    • References

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